Nested polymerase chain reaction for diagnostic and genotyping of chlamydia

evaluation of multiplex nested polymerase chain reaction for routine hepatitis c virus genotyping in egyptian patients

Evaluation of Multiplex Nested Polymerase Chain Reaction for Routine Hepatitis C Virus Genotyping in Egyptian Patients

BACKGROUND At least six HCV (hepatitis C virus) genotypes are unequally distributed worldwide. HCV genotyping guides the selection of treatment regimens and provides important epidemiological markers that enable the outbreak source to be traced and the spread of disease to be controlled. In Egypt, there is an increasing need for cost-effective, fast, and easily performable HCV genotyping assays...

One-tube nested polymerase chain reaction for detection of Chlamydia trachomatis.

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.

Rhesus D genotyping using polymerase chain reaction.

Sensitive detection and typing of Chlamydia trachomatis using nested polymerase chain reaction.

OBJECTIVES A method based on a nested polymerase chain reaction (PCR) was developed to detect and to type Chlamydia trachomatis from low titre samples by amplifying a large portion of the major outer membrane protein gene. The sensitivity of this procedure was evaluated in urogenital clinical samples in comparison with culture. SPECIMENS A series of 787 urogenital specimens, including 37 (4.7...